Credit Unit System
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1. Course Title:
Analytical Biochemistry
2. Course Code:
BCH4032
3. Course Aims & Objectives:
To introduce to students the basic principles of techniques used routinely in modern biochemistry and molecular biology.
On completion of this course, students should be able to:
- demonstrate an understanding on the basic principles and current status of analytical biochemistry;
- critically evaluate recent publications in research and development of analytical biochemistry;
- apply conventional bioanalytical techniques to chemical and biochemical research when necessary.
4. Units: 4
5. Level: B4 (This course will also be offered to postgraduate students.)
6. Medium of Instruction: English
7. Keyword Syllabus:
- General principles of biochemical investigation
In vivo and in vitro studies. Physiological solutions. Cell and tissue culture. Centrifugation.
- Nucleic acids and proteins
Structure and functions of nucleic acids. Replication, transcription, and translation. Protein structure. Enzymes.
- Isotopic and non-isotopic labellings
Labeling and detection of radioisotopes. Luminescence and chemiluminesence labels. Enzymatic labels.
- Immunochemical techniques
General principles. Antibody production. Immunoprecipitation. Immonoassays. ELISA. Immunohistochemistry.
- Molecular biology techniques
Synthesis and sequencing of DNA. Enzymatic manipulation of gene. Gene cloning and expression. PCR.
- Protein and enzyme techniques
Protein purification. Enzyme kinetics. Enzyme assays. Protein-ligand binding studies. Immobilized enzymes.
- Chromatographic and electrophoretic techniques
Adsorption, partition, ion-exchange, size-exclusion, and affinity LC’s. Agarose gel electrophoresis. PAGE. Capillary electrophoresis.
- Microscopic techniques
Fluorescence microscope. SEM and TEM. STM and AFM.
- Spectroscopic and electrochemical techniques
UV-visible. Spectrofluorimetry. Circular dichroism. MS. NMR. Redox enzymes. Oxygen and pH electrodes. Biosensors.
8. Teaching pattern:
Duration of course: 1 semester
Suggested lecture/tutorial/laboratory mix:
Lectures 39 H
Tutorials 13 H
9. Assessment pattern:
Examination duration: 3 H Percentage of coursework, examination, etc.: Examination 70% Course work 30% All students must satisfy the following "Minimum Passing Requirement" for BCH courses:
"A minimum of 30% in coursework as well as in examination, in addition to a minimum of 40% in coursework and examination taken together".
10. Pre-requisites:
BCH2004 Principles of Analytical Chemistry
11. Pre-cursor:
BCH2003 Biochemistry
12. Equivalent Courses:
BCH2374 Analytical Biochemistry (from the "old" curriculum)
13. Booklist:
Please consult your lecturer.
